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SLC3A2
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2DH2, 2DH3
Identifiers
Aliases	SLC3A2, 4F2, 4F2HC, 4T2HC, CD98, CD98HC, MDU1, NACAE, solute carrier family 3 member 2
External IDs	OMIM: 158070; MGI: 96955; HomoloGene: 1795; GeneCards: SLC3A2; OMA:SLC3A2 - orthologs
Gene location (Human) Chr. Chromosome 11 (human) Band 11q12.3 Start 62,856,004 bp End 62,888,880 bp
Gene location (Mouse) Chr. Chromosome 19 (mouse) Band 19 A|19 5.44 cM Start 8,684,246 bp End 8,700,733 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in islet of Langerhans ventricular zone stromal cell of endometrium right lobe of thyroid gland left lobe of thyroid gland ganglionic eminence upper lobe of left lung skin of abdomen canal of the cervix skin of leg Top expressed in epithelium of lens choroid plexus of fourth ventricle submandibular gland parotid gland calvaria spermatocyte stroma of bone marrow right kidney neural layer of retina lip More reference expression data BioGPS More reference expression data
Gene ontology Molecular function calcium:sodium antiporter activity neutral amino acid transmembrane transporter activity catalytic activity protein binding double-stranded RNA binding RNA binding cadherin binding Cellular component integral component of membrane membrane melanosome plasma membrane cell surface apical plasma membrane extracellular exosome nucleus cytosol Biological process response to exogenous dsRNA cell growth amino acid transport leukocyte migration calcium ion transport tryptophan transport sodium ion transmembrane transport L-alpha-amino acid transmembrane transport transport carbohydrate metabolic process L-leucine import across plasma membrane Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 6520 17254 Ensembl ENSG00000168003 ENSMUSG00000010095 UniProt P08195 P10852 RefSeq (mRNA) NM_001012661 NM_001012662 NM_001012663 NM_001012664 NM_001013251 NM_002394 NM_001161413 NM_008577 RefSeq (protein) NP_001012680 NP_001012682 NP_001013269 NP_002385 NP_001154885 NP_032603 Location (UCSC) Chr 11: 62.86 – 62.89 Mb Chr 19: 8.68 – 8.7 Mb PubMed search
Wikidata
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4F2 cell-surface antigen heavy chain is a protein that in humans is encoded by the SLC3A2 (solute carrier family 3 member 2) gene.

SLC3A2 comprises the heavy subunit of the large neutral amino acid transporter (LAT1) that is also known as CD98 (cluster of differentiation 98).

Function

SLC3A2 is a member of the solute carrier family and encodes a cell surface, transmembrane protein with an alpha-amylase domain. The protein exists as the heavy chain of a heterodimer, covalently bound through di-sulfide bonds to one of several possible light chains. It associates with integrins and mediates integrin-dependent signaling related to normal cell growth and tumorigenesis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

LAT1 is a heterodimeric membrane transport protein that preferentially transports neutral branched (valine, leucine, isoleucine) and aromatic (tryptophan, tyrosine, phenylalanine) amino acids. LAT is highly expressed in brain capillaries (which form the blood brain barrier) relative to other tissues.

A functional LAT1 transporter is composed of two proteins encoded by two distinct genes:

• 4F2hc/CD98 heavy subunit protein encoded by the SLC3A2 gene (this gene)
• CD98 light subunit protein encoded by the SLC7A5 gene

Interactions

SLC3A2 has been shown to interact with SLC7A7.

Additionally, SLC3A2 is a constituent member of the system xc- cystine/glutamate antiporter, complexing with SLC7A11.

See also

• Heterodimeric amino acid transporter

References

1. ^ GRCh38: Ensembl release 89: ENSG00000168003 – Ensembl, May 2017
2. ^ GRCm38: Ensembl release 89: ENSMUSG00000010095 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Teixeira S, Di Grandi S, Kühn LC (August 1987). "Primary structure of the human 4F2 antigen heavy chain predicts a transmembrane protein with a cytoplasmic NH2 terminus". J Biol Chem. 262 (20): 9574–80. doi:10.1016/S0021-9258(18)47972-0. PMID 3036867.
6. ^ "Entrez Gene: SLC3A2 solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2".
7. Kucharzik T, Lugering A, Yan Y, Driss A, Charrier L, Sitaraman S, Merlin D (2005). "Activation of epithelial CD98 glycoprotein perpetuates colonic inflammation". Lab. Invest. 85 (7): 932–41. doi:10.1038/labinvest.3700289. PMID 15880135. S2CID 19733993.
8. Lemaître G, Gonnet F, Vaigot P, Gidrol X, Martin MT, Tortajada J, Waksman G (2005). "CD98, a novel marker of transient amplifying human keratinocytes". Proteomics. 5 (14): 3637–45. doi:10.1002/pmic.200401224. PMID 16097038. S2CID 31954787.
9. ^ Boado RJ, Li JY, Nagaya M, Zhang C, Pardridge WM (1999). "Selective expression of the large neutral amino acid transporter at the blood–brain barrier". Proc. Natl. Acad. Sci. U.S.A. 96 (21): 12079–84. Bibcode:1999PNAS...9612079B. doi:10.1073/pnas.96.21.12079. PMC 18415. PMID 10518579.
10. Palacín M, Kanai Y (2004). "The ancillary proteins of HATs: SLC3 family of amino acid transporters". Pflügers Arch. 447 (5): 490–494. doi:10.1007/s00424-003-1062-7. PMID 14770309. S2CID 25808108.
11. Verrey F, Closs EI, Wagner CA, Palacin M, Endou H, Kanai Y (2004). "CATs and HATs: the SLC7 family of amino acid transporters" (PDF). Pflügers Arch. 447 (5): 532–542. doi:10.1007/s00424-003-1086-z. PMID 14770310. S2CID 11670040.
12. Pfeiffer R, Rossier G, Spindler B, Meier C, Kühn L, Verrey F (January 1999). "Amino acid transport of y+L-type by heterodimers of 4F2hc/CD98 and members of the glycoprotein-associated amino acid transporter family". EMBO J. 18 (1): 49–57. doi:10.1093/emboj/18.1.49. PMC 1171101. PMID 9878049.

Further reading

• Rochelle JM, Watson ML, Oakey RJ, Seldin MF (1992). "A linkage map of mouse chromosome 19: definition of comparative mapping relationships with human chromosomes 10 and 11 including the MEN1 locus". Genomics. 14 (1): 26–31. doi:10.1016/S0888-7543(05)80278-2. PMID 1358795.
• Michalak M, Quackenbush EJ, Letarte M (1986). "Inhibition of Na+/Ca2+ exchanger activity in cardiac and skeletal muscle sarcolemmal vesicles by monoclonal antibody 44D7". J. Biol. Chem. 261 (1): 92–5. doi:10.1016/S0021-9258(17)42436-7. PMID 2416754.
• Gottesdiener KM, Karpinski BA, Lindsten T, Strominger JL, Jones NH, Thompson CB, Leiden JM (1989). "Isolation and structural characterization of the human 4F2 heavy-chain gene, an inducible gene involved in T-lymphocyte activation". Mol. Cell. Biol. 8 (9): 3809–19. doi:10.1128/mcb.8.9.3809. PMC 365439. PMID 3265470.
• Lindsten T, June CH, Thompson CB, Leiden JM (1989). "Regulation of 4F2 heavy-chain gene expression during normal human T-cell activation can be mediated by multiple distinct molecular mechanisms". Mol. Cell. Biol. 8 (9): 3820–6. doi:10.1128/mcb.8.9.3820. PMC 365440. PMID 3265471.
• Quackenbush E, Clabby M, Gottesdiener KM, Barbosa J, Jones NH, Strominger JL, Speck S, Leiden JM (1987). "Molecular cloning of complementary DNAs encoding the heavy chain of the human 4F2 cell-surface antigen: a type II membrane glycoprotein involved in normal and neoplastic cell growth". Proc. Natl. Acad. Sci. U.S.A. 84 (18): 6526–30. Bibcode:1987PNAS...84.6526Q. doi:10.1073/pnas.84.18.6526. PMC 299110. PMID 3476959.
• Lumadue JA, Glick AB, Ruddle FH (1988). "Cloning, sequence analysis, and expression of the large subunit of the human lymphocyte activation antigen 4F2". Proc. Natl. Acad. Sci. U.S.A. 84 (24): 9204–8. doi:10.1073/pnas.84.24.9204. PMC 299721. PMID 3480538.
• Posillico JT, Srikanta S, Eisenbarth G, Quaranta V, Kajiji S, Brown EM (1987). "Binding of monoclonal antibody (4F2) to its cell surface antigen on dispersed adenomatous parathyroid cells raises cytosolic calcium and inhibits parathyroid hormone secretion". J. Clin. Endocrinol. Metab. 64 (1): 43–50. doi:10.1210/jcem-64-1-43. PMID 3782435.
• Hemler ME, Strominger JL (1982). "Characterization of antigen recognized by the monoclonal antibody (4F2): different molecular forms on human T and B lymphoblastoid cell lines". J. Immunol. 129 (2): 623–8. doi:10.4049/jimmunol.129.2.623. PMID 6177771. S2CID 42283583.
• Peters PG, Kamarck ME, Hemler ME, Strominger JL, Ruddle FH (1983). "Genetic and biochemical characterization of a human surface determinant on somatic cell hybrids: the 4F2 antigen". Somatic Cell Genet. 8 (6): 825–34. doi:10.1007/BF01543022. PMID 6187076. S2CID 19788137.
• Francke U, Foellmer BE, Haynes BF (1983). "Chromosome mapping of human cell surface molecules: monoclonal anti-human lymphocyte antibodies 4F2, A3D8, and A1G3 define antigens controlled by different regions of chromosome 11". Somatic Cell Genet. 9 (3): 333–44. doi:10.1007/BF01539142. PMID 6190235. S2CID 22972681.
• Haynes BF, Hemler ME, Mann DL, Eisenbarth GS, Shelhamer J, Mostowski HS, Thomas CA, Strominger JL, Fauci AS (1981). "Characterization of a monoclonal antibody (4F2) that binds to human monocytes and to a subset of activated lymphocytes". J. Immunol. 126 (4): 1409–14. doi:10.4049/jimmunol.126.4.1409. PMID 7204970. S2CID 42367568.
• Ohgimoto S, Tabata N, Suga S, Nishio M, Ohta H, Tsurudome M, Komada H, Kawano M, Watanabe N, Ito Y (1995). "Molecular characterization of fusion regulatory protein-1 (FRP-1) that induces multinucleated giant cell formation of monocytes and HIV gp160-mediated cell fusion. FRP-1 and 4F2/CD98 are identical molecules". J. Immunol. 155 (7): 3585–92. doi:10.4049/jimmunol.155.7.3585. PMID 7561057. S2CID 8787778.
• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Andersson B, Wentland MA, Ricafrente JY, Liu W, Gibbs RA (1996). "A "double adaptor" method for improved shotgun library construction". Anal. Biochem. 236 (1): 107–13. doi:10.1006/abio.1996.0138. PMID 8619474.
• Ohgimoto S, Tabata N, Suga S, Tsurudome M, Kawano M, Nishio M, Okamoto K, Komada H, Watanabe N, Ito Y (1996). "Regulation of human immunodeficiency virus gp160-mediated cell fusion by antibodies against fusion regulatory protein 1". J. Gen. Virol. 77 (11): 2747–56. doi:10.1099/0022-1317-77-11-2747. PMID 8922468.
• Courseaux A, Grosgeorge J, Gaudray P, Pannett AA, Forbes SA, Williamson C, Bassett D, Thakker RV, Teh BT, Farnebo F, Shepherd J, Skogseid B, Larsson C, Giraud S, Zhang CX, Salandre J, Calender A (1997). "Definition of the minimal MEN1 candidate area based on a 5-Mb integrated map of proximal 11q13. The European Consortium on Men1, (GENEM 1; Groupe d'Etude des Néoplasies Endocriniennes Multiples de type 1)". Genomics. 37 (3): 354–65. doi:10.1006/geno.1996.0570. PMID 8938448.
• Yu W, Andersson B, Worley KC, Muzny DM, Ding Y, Liu W, Ricafrente JY, Wentland MA, Lennon G, Gibbs RA (1997). "Large-Scale Concatenation cDNA Sequencing". Genome Res. 7 (4): 353–8. doi:10.1101/gr.7.4.353. PMC 139146. PMID 9110174.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Tabata N, Ido M, Suga S, Ohgimoto S, Tsurudome M, Kawano M, Nishio M, Watanabe N, Okamoto K, Komada H, Sakurai M, Ito Y (1998). "Protein tyrosine kinase activation provides an early and obligatory signal in anti-FRP-1/CD98/4F2 monoclonal antibody induced cell fusion mediated by HIV gp160". Med. Microbiol. Immunol. 186 (2–3): 115–23. doi:10.1007/s004300050053. PMID 9403839. S2CID 7693336.

External links

• Large+Neutral+Amino+Acid-Transporter+1 at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
• Amino+Acid+Transport+System+L at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

This article incorporates text from the United States National Library of Medicine, which is in the public domain.

• Genes on human chromosome 11
• Solute carrier family