Bothwell is a Scottish surname. Notable people with the surname include:
- Dorr Bothwell (1902–2000), American artist
- Fluff Bothwell, American football player
- Francis Bothwell of Edinburgh, 16th-century Scottish merchant, landowner, judge and member of the Scottish Parliament
- John Bothwell (bishop) (1926–2014), Canadian Anglican bishop and writer
- Robert Bothwell (born 1944), Canadian historian and writer
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