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Route 216 is a two-lane east/west highway in Quebec, Canada, which starts in Sainte-Catherine-de-Hatley in the Estrie region at the junction of Route 108 and ends in Sainte-Perpétue in Chaudière-Appalaches at the junction of Route 204.
Route 216 follows mostly a northeast/southwest course, and it is not a busy highway as it mostly links small villages between themselves in the backroads of the Appalachians. The only two major towns along the way are Sherbrooke and Sainte-Marie. Between Ham-Nord (junction with Route 161) and Saint-Jacques-le-Majeur-de-Wolfestown (junction with Route 263), the road is unpaved.