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Sulforhodamine 101

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Red fluorescent dye
Sulforhodamine 101
Identifiers
CAS Number
3D model (JSmol)
Beilstein Reference 3582548
ECHA InfoCard 100.056.491 Edit this at Wikidata
PubChem CID
UNII
CompTox Dashboard (EPA)
SMILES
  • S(C(C=C(S(O)(=O)=O)C=C1)=C1C2=C(C=C3C4=C5CCCN4CCC3)C5=C6=C(CCCN7CCC8)C7=C8C=C62)(=O)=O
Properties
Chemical formula C31H30N2O7S2
Molar mass 606.71 g·mol
Except where otherwise noted, data are given for materials in their standard state (at 25 °C , 100 kPa). checkY verify (what is  ?) Infobox references
Chemical compound

Sulforhodamine 101 (SR101) is a red fluorescent dye. In neurophysiological experiments which comprise calcium imaging methods, it can be used for a counterstaining of astrocytes to be able to analyze data from neurons separately. However, in addition to labeling astrocytes, SR101 labels myelinating oligodendrocytes. SR101 has been reported to affect excitability of neurons and should therefore be used with caution.

A sulfonyl chloride derivative of sulforhodamine 101, known as Texas Red, is used for conjugation to a number of functional groups, especially primary amines.

References

  1. Nimmerjahn, A.; Kirchhoff, F.; Kerr, J.N.; Helmchen, F. (2004). "Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo". Nature Methods. 1 (1): 31–7. doi:10.1038/nmeth706. PMID 15782150. S2CID 2073853.Closed access icon
  2. Hill, R.A.; Grutzendler, J. (2014). "In vivo imaging of oligodendrocytes with sulforhodamine 101". Nature Methods. 11 (11): 1081–1082. doi:10.1038/nmeth.3140. PMC 4539948. PMID 25357236.Closed access icon
  3. Kang, J.; Kang N.; Yu, Y.; Zhang, J.; et al. (2010). "Sulforhodamine 101 induces long-term potentiation of intrinsic excitability and synaptic efficacy in hippocampal CA1 pyramidal neurons". Neuroscience. 169 (4): 1601–1609. doi:10.1016/j.neuroscience.2010.06.020. PMC 2918738. PMID 20600669.
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